How did we perform preliminary screening for the phytochemical classes?
While performing the laboratory tasks for my dissertation work, I searched many articles and books to have a reliable protocol which would be easy to follow. However, I found either very standard books which compiled the test methods in lengthy literature or research articles mentioning test procedures only briefly; easy to understand but not easy to follow. At last, I consulted protocols by three authors (which are named below) and did according to the state of our laboratory. In this post, I am going to share the information about how we (me and my junior fellow) detected the presence of phytochemical classes in orchid sample extract s. The aim of this post is to provide reliable and easy to follow protocol for undergraduate and graduate students who wish to detect phytochemical classes in sample plant extracts. Basically, we followed protocols established by Habourne (1998), Trease and Evans (1983) with minor modifications according t...