Plantenthusiast

Estimation of total polyphenols content Figure1. The appearance of bluish-black colour after the addition of reagents on sample plant extracts during the estimation of total polyphenols content.  We estimated  total polyphenol content  by a standard protocol (Singleton et al . 1999 and Stankovic 2011) with slight modification. Briefly, we  separately mixed  each plant extract (0.5 ml; 1 mg/ml)  with the Folin-Ciocalteu phenol reagent (2.5 ml; 10%) and aqueous sodium bicarbonate solution (2.5 ml; 7.5%). Then, the reaction mixture was allowed to stand for about 45 minutes and the absorbance was measured at 765 nm using the UV- spectrophotometer (Thermo Fisher Scientific, Genesystem -10.5).The calibration curve was plotted using gallic acid as standard in ethanol (absolute) using the concentration series of 25, 50, 75, and 100 µg/ml. Here, the reagent mixture with the  same volume of ethanol instead of plant extract solution was used as a blank. On the basis of this calibration