How we performed the estimation of total flavonoid content ?
This time I am sharing a method for the estimation of total flavonoid content in our experiments. We Flowchart. the process of estimating total flavonoids content. followed a previously done standard protocol (Quettier et al. 2000 and Stankovic 2011) with slight modification. Firstly, we mixed e ach plant extract (2 ml; 0.5 mg/ml) separately with aluminium chloride solution (1 ml; 2 %). Then, we allowed the reaction mixture to stand for an hour at room temperature. Again, we measured the absorbance of the mixture at 415 nm using the UV-spectrophotometer. We obtained calibration curve (see Fig 1.) using quercetin solution series (25, 50 75, and 100 µg/ml) as a standard. Here, we used the reagent mixture with the same volume of ethanol instead of plant extract solution as a blank. Thus, the obtained curve was calibrated to estimate the concentration of total flavonoid in each plant extract. The total flavonoids content was expressed in terms of the milligram of qu