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How we performed the estimation of total flavonoid content ?

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This time I am sharing a method for the estimation of total flavonoid content in our experiments. We Flowchart. the process of estimating total flavonoids content. followed a previously done standard protocol  (Quettier  et al.  2000 and Stankovic 2011)  with slight modification.  Firstly, we  mixed  e ach plant extract (2 ml; 0.5 mg/ml) separately  with aluminium chloride solution (1 ml; 2 %). Then, we allowed the reaction mixture to stand for an hour at room temperature. Again, we measured the absorbance of the mixture at 415 nm using the UV-spectrophotometer. We  obtained  calibration curve (see Fig 1.) using quercetin solution series (25, 50 75, and 100 µg/ml) as a standard. Here, we used the reagent mixture with the same volume of ethanol instead of plant extract solution as a blank. Thus, the obtained curve was calibrated to estimate the concentration of total flavonoid in each plant extract. The total flavonoids content was expressed in terms of the milligram of qu